根据对苹果器官的致病性差异可将苹果炭疽叶枯病划分为两种致病类型：苦腐型（ABR pathotype）和叶枯型（GLS pathotype）。
苦腐型仅侵染近成熟果实，引起典型腐烂症状，但不侵染叶片，对品种无选择性，该类病害通常称为苦腐病（apple bitter rot，ABR），或苹果炭疽病（Southworth 1891；Sutton 1990）。
苹果
叶枯型能侵染叶片和幼果，对品种有严格的选择性，主要危害嘎拉系、金冠系和元帅系苹果品种，引起炭疽叶枯病（Leite et al. 1988；Sutton & Sanhueza 1998）。
本研究通过基因功能证明Cfcyp450是一个叶枯型菌株关键致病基因。同源性比对显示C. fructicola叶枯型基因Cfcyp450与C. aenigma叶枯型基因相似度为100%，为同源基因，但在C. fructicola苦腐型菌株基因组中不存在该基因。
苹果
此外Cfcyp450与已知*草烟**内生真菌Sodiomyces alkalinus F11和拟南芥内生真菌Dactylonectria macrodidyma同源性相对较高（刘宏玉 2014； Grum-Grzhimaylo et al. 2018；Habibi & Ghaderi 2020；Kuvarina et al. 2021），与刺盘孢属真菌亲缘关系较远，同源性低。
这些结果暗示苦腐型菌株可能通过一些内生真菌的基因水平转移获得能侵染叶片的独特基因，从而变成叶枯型菌株，该假设有待进一步的试验验证。
真菌的P450基因为一类重要致病基因，可通过参与次级代谢产物的合成来影响真菌的致病 性。
最近对植物病原菌CYP基因的功能分析中发现一些新的CYP基因，涉及毒性、无性发育和有性发育以及外源生物的降解。灰葡萄孢Botrytis cinerea P450单加氧酶基因bcbot1在植物中表达，对B. cinerea的侵染发挥重要作用（Verena et al. 2005）。
苹果
大丽轮枝菌Verticillium dahliae的T-DNA突变体M01C06致病性下降，其单加氧酶基因VdCYP1是关键致病相关因子，可能通过参与大丽轮枝菌的次级代谢影响致病过程（Zhang et al. 2016）。
稻瘟菌Magnaporthe oryzae中鉴定到MOMCP1，通过影响附着胞的形成而增强致病力( Wangetal.2019)。尖豌豆专化型Foxysporum f.sp.pisi 晚豆素脱甲基酶FOPDA能够对植保索豆素进行解毒促进其侵染( Georgeet al. 1998;Coleman et al.2011)。
本研究显示果生刺盘中Cfcyp450基因含有保守的CYP450结构域，通过影响附着胞的形成及穿透而影响致病力，是否参与解毒作用、次级代谢产物的合成来影响真菌的致病性尚有待进一步研究。
果生刺盘抱为半活体营养菌。分生抱子萌发产生芽管，芽管末端膨大形成附着胞，附着胞通过产生侵染钉穿透寄主角质层和表皮细胞，分化形成活体初生侵染菌丝，初生侵染菌丝随后分化形成次生侵染菌丝，以死体营养方式在组织内扩展( Crusius etal. 2002; Shang etal.2020)。
果生刺盘抱为一种寄主范围非常广泛的病原菌，近年来对其致病基因功能进行了许多研究。油茶果生刺盘抱 bZIP 转录因子 CfHacI 和自噬相关因子 CfAtg8 参与调控 ccticola 的生长发育、附着胞的形成、致病力及响应外界渗透压的胁迫过程(姚权等 2019: 郭源等 2020)。
果炭疽叶枯病病原果生刺盘抱转录因子 CfStel2 基因缺失后其分生子萌发率、附着胞形成率显著下降并丧失对嘎拉叶片和果实的致病性(Liu etal.2020)。C. ucticola 的 MAPK 基因 CfPMKI敲除突变体不能分化形成附着胞，因此不能穿透角质层，致病力显著下降 ( Liang et al 2019)。
C.fructicola 的 MADS-box 转录因子 CMcmI调控致病性分生抱子萌发和性发育(Liu etal.2022)。本研究中发现苹果果生刺盘抱 Cfcyp450 缺失突变体产抱量下降、附着胞的萌发和穿透降低，致病力显著减弱，我们推测 Cfcyp450 通过影响果生刺盘抱附着胞的萌发和穿透，导致其致病力减弱。有关 Cfcyp450对致病性的具体机制还有待进一步的探索。
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